Docente
Ana Isabel Gusmão Lima

Resume

Ana Lima is an assistant professor at Faculty of Veterinary Medicine, Lusofona University, Lisbon, as well as a member of the Research Unit Linking Landscape, Environment, Agriculture and Food (LEAF) at the Higher Institute of Agronomy (ISA), University of Lisbon. She completed her PhD degree in 2010 and later developed her Postdoc in the area of food anticancer bioactivities at ISA, where from 2015 to 2018 she shared the coordination of the Plants for Health and Disease group in ISA. From 2019 to 2021 she was the senior coordinator of the Science research department at a non-profit national institution. Currently she is part of the research committee at the Faculty of Veterinary Medicine,  Lusófona University. Her expertise resides mainly on proteomics and metabolomics, with a particular focus on bioactive compounds from food and plants for health, mainly focusing on inflammation and cancer preventive/ curative diets and natural alternatives to antibiotics and food disinfectants. She also works in the area of nutraceutical incorporation in functional foods and their impact in digestion and the gut microbiota. She has developed solid protocols for isolating and testing bioactive compounds in foods, mainly proteins/peptides, as well as an array of protocols for anti-inflammatory and anticancer activities using in vitro and in vivo tests. She has broad experience in cell culture and anticancer and anti-proliferation assays as well and on digestion models. She has participated in 15 projects both national and European and supervised 4, one of which FCT-funded.  She has supervised 4 PhD students, 1 post-doc, 12 MSC  and dozens of undergraduate students. She has 47 peer-reviewed publications, including book chapters and scientific papers, and has co- edited a book. She has received the Born from Knowledge Award from Food Nutrition in 2018 and holds already 3 international patents from her discoveries.

Graus

• Doutoramento
  Mestrado em Toxicologia - ano curricular
• Mestrado
  Mestrado em Toxicologia - ano curricular
• Mestrado
  Mestrado em Toxicologia - ano curricular
• Licenciatura
  Licenciatura em Biologia
• Mestrado
  Mestrado em Toxicologa
• Doutoramento
  Doutoramento

Publicações

Artigo em revista

• 2023-02, Milk's Bioactive Peptides: Are We Disregarding Nature's Miracle?, Novel Techniques in Nutrition and Food Science
• 2022-12-14, Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression, Cancers
• 2022-08-17, Applications of Contrast-Enhanced Ultrasound in Splenic Studies of Dogs and Cats, Animals
• 2022-07-14, New Alternatives to Milk From Pulses: Chickpea and Lupin Beverages With Improved Digestibility and Potential Bioactivities for Human Health, Frontiers in Nutrition
• 2022-05, Lupin Protein Concentrate as a Novel Functional Food Additive That Can Reduce Colitis-Induced Inflammation and Oxidative Stress, Nutrients
• 2022-03-31, Applications of Essential Oils as Antibacterial Agents in Minimally Processed Fruits and Vegetables—A Review, Microorganisms
• 2021-12, Lupinus albus Protein Components Inhibit MMP-2 and MMP-9 Gelatinolytic Activity In Vitro and In Vivo, International Journal of Molecular Sciences
• 2021-10, Sourdough Fermentation as a Tool to Improve the Nutritional and Health-Promoting Properties of Its Derived-Products, Fermentation
• 2021-10, Extended Cheese Whey Fermentation Produces a Novel Casein-Derived Antibacterial Polypeptide That Also Inhibits Gelatinases MMP-2 and MMP-9, International Journal of Molecular Sciences
• 2021-10, Combination of Trans-Resveratrol and e-Viniferin Induces a Hepatoprotective Effect in Rats with Severe Acute Liver Failure via Reduction of Oxidative Stress and MMP-9 Expression, Nutrients
• 2021, Mota J, Ferreira RB, Raymundo A, Lima A. (2021). Development of a lupin protein concentrate as a nutraceutical delivery system for addition to cancer-preventing foods., Foods
• 2021, M. I. S. Santos, A. I. G. Lima, J. Mota, P. Rebelo, R. M. S. B. Ferreira, L. Pedroso, I. Sousa (2021). Extended fermentation produces a broad-range antibacterial peptide that also targets matrix metalloproteases related to inflammation and cancer. , Journal of Advanced Research
• 2021, An Up-Scalable and Cost-Effective Methodology for Isolating a Polypeptide Matrix Metalloproteinase-9 Inhibitor from Lupinus albus Seeds, Foods
• 2021, Actinic keratosis treated by topical Aloe barbadensis Mill. (Aloe Vera) leaf gel, Academia letters
• 2020-12, Differential inhibition of gelatinase activity in human colon adenocarcinoma cells by Aloe vera and Aloe arborescens extracts, BMC Complementary Medicine and Therapies
• 2020-10-04, Glycemic Response and Bioactive Properties of Gluten-Free Bread with Yoghurt or Curd-Cheese Addition, Foods
• 2020-08-05, Lupin Seed Protein Extract Can Efficiently Enrich the Physical Properties of Cookies Prepared with Alternative Flours, Foods
• 2020, Deflamin-an-edible-antiinflammatory-protein-isolated-from-legume-seeds, Journal of Nutraceutics and Food Science
• 2020, A Novel Way for Whey: Cheese Whey Fermentation Produces an Effective and Environmentally-Safe Alternative to Chlorine, Eco Novel Food and Feed
• 2019-10, Reduction of inflammation and colon injury by a Pennyroyal phenolic extract in experimental inflammatory bowel disease in mice, Biomedicine & Pharmacotherapy
• 2019-06-22, New Lectins from Mediterranean Flora. Activity against HT29 Colon Cancer Cells, International Journal of Molecular Sciences
• 2019-06-06, Reduction of Inflammation and Colon Injury by a Spearmint Phenolic Extract in Experimental Bowel Disease in Mice, Medicines
• 2017-10-09, Essential oils as antibacterial agents against food-borne pathogens: Are they really as useful as they are claimed to be?, Journal of Food Science and Technology
• 2017-08, Dyospiros kaki phenolics inhibit colitis and colon cancer cell proliferation, but not gelatinase activities, The Journal of Nutritional Biochemistry
• 2017-02-27, Proteins in Soy Might Have a Higher Role in Cancer Prevention than Previously Expected: Soybean Protein Fractions Are More Effective MMP-9 Inhibitors Than Non-Protein Fractions, Even in Cooked Seeds, Nutrients
• 2016-08, Preliminary Study on the Effect of Fermented Cheese Whey on Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella Goldcoast Populations Inoculated onto Fresh Organic Lettuce, Foodborne Pathogens and Disease
• 2016-04, Legume seeds and colorectal cancer revisited: Protease inhibitors reduce MMP-9 activity and colon cancer cell migration, Food Chemistry
• 2012, Looking for suitable biomarkers in benthic macroinvertebrates inhabiting coastal areas with low metal contamination: Comparison between the bivalve Cerastoderma edule and the Polychaete Diopatra neapolitana, Ecotoxicology and Environmental Safety
• 2012, Cd-induced signaling pathways in plants: Possible regulation of PC synthase by protein phosphatase 1, Environmental and Experimental Botany
• 2011, Health concerns of consuming cockles (Cerastoderma edule L.) from a low contaminated coastal system, Environment International
• 2010, Sensitivity of biochemical markers to evaluate cadmium stress in the freshwater diatom Nitzschia palea (Kützing) W. Smith, Aquatic Toxicology
• 2009, Mercury intracellular partitioning and chelation in a salt marsh plant, Halimione portulacoides (L.) Aellen: Strategies underlying tolerance in environmental exposure, Chemosphere
• 2009, Accumulation, distribution and cellular partitioning of mercury in several halophytes of a contaminated salt marsh, Chemosphere
• 2006, The importance of glutathione in oxidative status of Rhizobium leguminosarum biovar viciae under Cd exposure, Enzyme and Microbial Technology
• 2006, Screening possible mechanisms mediating cadmium resistance in Rhizobium leguminosarum bv. viciae isolated from contaminated Portuguese soils, Microbial Ecology
• 2006, Heavy metal toxicity in Rhizobium leguminosarum biovar viciae isolated from soils subjected to different sources of heavy-metal contamination: Effects on protein expression, Applied Soil Ecology
• 2006, Glutathione-mediated cadmium sequestration in Rhizobium leguminosarum, Enzyme and Microbial Technology
• 2006, Cadmium uptake in PEA plants under environmentally-relevant exposures: The risk of food-chain transfer, Journal of Plant Nutrition
• 2006, Cadmium detoxification in roots of Pisum sativum seedlings: Relationship between toxicity levels, thiol pool alterations and growth, Environmental and Experimental Botany
• 2005, Cadmium tolerance plasticity in Rhizobium leguminosarum bv. viciae: Glutathione as a detoxifying agent, Canadian Journal of Microbiology

Capítulo de livro

• 2023, A Realistic View of Essential Oils as Antibacterial Agents for Ready-to-Eat Fruits and Vegetables, Advances in Public Health , 1, 1, Academic reads
• 2021, Industrial waste whey as a low-cost, efficient, and environmentally safe disinfectant, with potential applications for minimally processed foodstuff., Current Approaches in Science and Technology Research. BP International, BP International. India.
• 2012, The influence of GSH on Rhizobium tolerance to cadmium, Toxicity of Heavy Metals to Legumes and Bioremediation, Springer-Verlag
• 2011, Metal signaling in plants: new possibilities for crop management under cadmium contaminated soils, Biomanagement of Metal Contaminated Soils, Springer-Verlag
• 2010, Mercury tolerance in higher plants: emerging views, Heavy Metal Stress Tolerance in Plants: Recent Advances in Physiology & Molecular Biology, Springer-Verlag
• 2008, Rhizobium leguminosarum isolated from agricultural ecosystems subjected to different climatic influences: the relation between genetic diversity, salt tolerance and nodulation efficiency, Soil Ecology Research Development, Nova Science Publishers Inc.
• 2006, The role of phytochelatins in cadmium detoxification in Pisum sativum L., Cadmium Stress in Plants, Springer-Verlag

Artigo em conferência

• 2022, Péptidos produzidos por fermentação por bactérias do ácido láctico como novas alternativas antimicrobianas e anti-inflamatórias, Microbiologia 2022

Poster em conferência

• 2021-10-22, Uso da norma ISO 9001:2008 para a implementação e certificação de um sistema de gestão de qualidade num centro de atendimento médico veterinário. , Medicina Interna sem segredos, Congresso Internacional Veterinário Montenegro
• 2021-06-28, Protective effect of stilbenes in rats with severe acute liver failure. A new role for grapevine.
• 2021, New alternatives to milk from pulses: digestibility and bioactivity, Agri-Food Ecosystem, Santarém (May, 26-27)
• 2021, New alternatives to milk from pulses: digestibility and bioactivity, Encontro Ciência 2021, Lisboa (June 28-30).
• 2021, Lupin Seed Protein Extract Can Enrich the Physical Properties of Cookies Prepared with Alternative Flours, Encontro Ciência 2021, Lisboa (June 28-30).
• 2021, Emerging new possibilities on bioactive whey peptides: extended fermentation produces a novel casein-derived peptide with broad-range antibacterial activities that also inhibits MMP-2 and MMP-9, Encontro Ciência 2021, Lisboa (June 28-30).
• 2021, Duarte CM, Mota J, Assunção R, Martins C, Ribeiro A, Lima A, Raymundo A, Nunes C, Boavida-Ferreira R, Sousa I. (2021). New alternatives to milk from pulses: digestibility and bioactivity. Agri-Food Ecosystem, , Encontro Ciência 2021, Lisboa (June 28-30).
• 2021, Anti-inflammatory and anticancer activities of a protein isolated from Lupinus seeds and its application in functional foods., Encontro Ciência 2021, Lisboa (June 28-30).
• 2018, Rheological properties evaluation of bread dough with different levels of fermented whey, AERC2018 - The Annual European Rheology Conference
• 2018, Pão enriquecido com soro de leite fermentado: uma alternativa sustentável., Food Bioactives and Health Conference
• 2018, Fermented cheese whey as a novel alternative to chlorine in lettuce disinfection, 5º Simpósio Nacional “Promoção de uma Alimentação Saudável e Segura – SPASS 2018
• 2018, Deflamin, an edible anti-inflammatory and anticancer protein isolated from legume seeds, Ciência 2018
• 2014-09, M. Biological potential of fermented whey: improving the isolation of bioactive peptides., 7th International Whey Conference
• 2014-04, Aloe species reduces colon cancer cell invasion through specific MMP-9 inhibition, Jornadas de Biologia.
• 2013-09, Antimicrobial peptides produced by whey fermentation, ISOPOL
• 2011-05-02, Avaliação da contaminação metálica em duas espécies de macroinvertebrados da ria de Aveiro por marcadores bioquímicos, Jornadas da Ria, Universidade de Aveiro
• 2007-11-26, Hg-induced oxidative stress in diferent salt marsh species, SOWETOX 2007 Soil and Wetland Toxicology
• 2007-11-26, Hg accumulation by different salt marsh species: potential use for phytoremediation, SOWETOX 2007 Soil and Wetland Toxicology International Meeting
• 2007-11-26, Are phytochelatins a mechanism for Hgphytoremediation in salt marshes?, SOWETOX 2007 Soil and Wetland Toxicology
• 2007-08, Calcium sensors are regulated by different stress signals, World Stress Conference
• 2007-07-24, The role of the B subunit of PP2B in plant stress signaling, Europhosphatases
• 2006-07, Uptake and distribution of mercury in a salt marsh plant, Atriplex patula (L.), International Symposium on Environmental Biotechnology
• 2006-07, Sulphide incorporation and time-maturation of PC-Cd complexes in Pisum sativum, International Symposium on Environmental Biotechnology
• 2006-07, DNA isolation from recalcitrant tissues of grapevine, FESPB 15th Congress on Plant Molecular Biology
• 2006-07, Cadmium and GSH absorption and exclusion during exposure to Cd in Rhizobium leguminosarum., International Symposium on Environmental Biotechnology
• 2005-09-18, Maturation of phytochelatincadmium complexes in roots of Pisum sativum L., IX Congresso Luso-Espanhol de Fisiologia Vegetal
• 2005, Identification of Cd-protein complexes in Cd tolerant Rhizobium leguminosarum strain, BioMicroworld, I Congress in Microbiology
• 2005, Heavy metal toxicity in Rhizobium leguminosarum biovar viciae isolated from soils neighbouring metalomechanics industries, BioMicroworld, I Congress in Microbiology
• 2005, Cadmium tolerance of Rhizobium leguminosarum bv. viciae isolated from Portuguese soils contaminated with heavy metals: mechanisms of tolerance., BioMicroworld, I Congress in Microbiology
• 2004, The relationship between thiol pool alterations, phytochelatin production and growth in Pisum sativum under different cadmium exposures, Simpósio de Nutrição Mineral em Plantas
• 2004, Metal tolerance in Rhizobium leguminosarum bv. viciae: an overview of possible tolerance mechanisms, In: X Simpósio de Nutrição Mineral em Plantas
• 2004, Involvement of Glutathione in Rhizobium leguminosarum heavy metal tolerance, XIV Congresso de Bíoquimica
• 2002-12-05, Protein profile alterations induced after cadmium exposure in Rhizobium leguminosarum, XIII Congresso Nacional de Bioquímica
• 2002-12-05, Effects of salinity on protein patterns of Rhizobium leguminosarum strains with different salt tolerances, XIII Congresso Nacional de Bioquímica
• 2002-12-05, Effects of Cadmium on Pisum sativum / Rhizobium leguminosarum symbiosis., XIII Congresso Nacional de Bioquímica

Patente

• SKOPOBIOTA, Blad & Bentochar: Plant treatment, PCT/EP2020/06574
• DEFLAMIN: Therapeutic protein, PCT/EP2017/07502
• DEFLAMIN: Therapeutic protein, PCT/EP2017/0