Part of this Programme
Level of Qualification|Semesters|ECTS
Bachelor | Semestral | 5
Year | Type of course unit | Language
2 |Mandatory |Português
Total of Working Hours | Duration of Contact (hours)
125 | 60
Recommended complementary curricular units
Prerequisites and co-requisites
Introduction to Biochemistry The water molecule Physicochemical properties of amino acids Proteins and their characterization Hemeproteins Enzymes Catalytic mechanisms Carbohydrates: Monosaccharides and polysaccharides; Glycoproteins. Lipids. Biological membranes (fluid mosaic model) and biological transport: passive, facilitated and active. Lipids. Biological membranes Nucleic acids (RNA and DNA): structure and function. Denaturation and renaturation of nucleic acids. The flow of genetic information: from gene to protein. Introduction to recombinant DNA technology: restriction endo-nucleases; DNA sequencing by the dideoxyribonucleotide addition method; Introduction to PCR; cloning vectors and expression vectors; directed mutagenesis. Introduction to PCR Introduction to bioenergetics. Major metabolic pathways (glycolysis and fermentation, gluconeogenesis, citric acid cycle): reactions and their regulation. Oxidative phosphorylation: electron transport chain and ATP synthesis
In the course of Biochemistry students are expected to acquire the fundamental knowledge of concepts related to the structure, properties and biological functions of the main classes of biomolecules such as carbohydrates, lipids, amino acids, peptides, proteins and nucleic acids. The study of major metabolic pathways and bioenergetics complement the fundamental aspects of biochemistry in general. These basic concepts are fundamental to the understanding and monitoring of other curricular units of the study plan. In the laboratory classes are introduced the basic techniques of Biochemistry, aiming to complete and apply the theoretical knowledge acquired.
Knowledge, abilities and skills to be acquired
The curricular unit of Biochemistry gives a preparation on general and fundamental concepts of this scientific area, allowing an interconnection with other specific areas, namely, Engineering, Food Sciences, Genetics, Biomedicine and others. The practical component of the discipline allows for preparation in the various techniques used in Biochemistry by providing the fundamental tools in preparation for future performance in several areas namely in the area of research and in the area of Biotechnology in general.
Teaching methodologies and assessment
Assessment: The final classification of the course results from the weighted average of the theoretical evaluation (60%) and the laboratory part (40%). Assessment Type Assessment only with final exam Registered Assessment components and amount of time allocated Description Type Hours Date of conclusion Attendance (estimated) Classes Assessment Type Assessment only with final exam Registered Assessment components and amount of time allocated Description Type Hours Date of conclusion Attendance Evaluation of the practical part = Continuous evaluation, based on scientific reports (75%) + discussion of the same (25%).
LEHNINGER PRINCIPLES OF BIOCHEMISTRY Nelson, D.L., & Cox, M.M. W.H. Freeman and Company, San Francisco, 5th ed. 2008
PRINCIPLES OF BIOCHEMISTRY Voet, D., Voet, J.G. & Pratt, C.W. John Wiley & Sons, Inc., New York, 3rd ed. 2008
BIOCHEMISTRY Lubert Stryer, W. H. Freeman and Company, San Francisco. 6th Ed. 2007
BIOQUÍMICA - ORGANIZAÇÃO MOLECULAR DA VIDA - Alexandre Quintas, Ana Ponces Freire, Manuel J. Halpern, LIDEL, 2008