Class Assisted Reproduction Techniques: Cryobiology

  • Presentation


    This curricular unit will allow the student to apply the theorical and practical knowledge acquired in previous CUs and corelated them with criopreservation of gametes and embryos.

  • Code


  • Syllabus



    Concepts of cryolbiology applied to protocols of:

    1. Sperm Cryopreservation
    2. Embryo cryopreservation
    3. Oocyte Cryopreservation


    Practices on the criopreservation and thawing of gametes and embrios.

  • Objectives


    At the end of this Course Unit (UC) the student should understand the concepts of cryobiology, namely the rationale for the use of permeant and non-permeant cryoprotectants and apply the knowledge acquired in understanding cryopreservation protocols. Students should also be able to understand the importance of the cryopreservation program in the dynamics of the assisted reproduction laboratory.

    At a practical level, the student should employ gamete and embryo manipulation techniques, acquired throughout the other curricular units, to the cryopreservation of gametes and embryos.

  • Teaching methodologies and assessment

    Teaching methodologies and assessment

    The master in Embryology and Human Reproduction has a theorical component, given in
    modules by guests specialized in the subjects of each module, among which are
    clinician, embryologists, national and international clinical directors of assisted
    reproductive (ART) centers, and/or representatives of Portuguese and European
    societies. Each teacher is asked to select a few scientific papers and/or clinical cases
    related to the subject they’ve teach so the students can prepare presentations and
    discussion of those, in the final evaluation of the module. Students are, this way,
    familiarized with discussion and interpretation of scientific papers and/or clinical cases as
    in the clinical environment.

    The practical component is given in the embryology laboratory of this University. Student practice
    all the techniques of in vitro fertilization (using animal models), in an environment identical to the clinical one.

  • References


    • Cryopreservation of Mammalian Gametes and Embryos (2017) Editors ZN Peter, CV Alex and A Agarwal. Humana Press, New York, NY. DOI:

    • Atlas of human embryology: from oocytes to preimplantation embryos.(2012)MC Magli; GM Jones; K Lundin; E Van den Abbeel and the Special Interest Group on Embryology – ESHRE. Human Reproduction 27, sup 1 – Oxford Press DOI 10.1093/humrep/des200

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