Part of this Programme
Level of Qualification|Semesters|ECTS
Bachelor; Master Degree | Semestral | 4
Year | Type of course unit | Language
2 |Mandatory |Português
Total of Working Hours | Duration of Contact (hours)
110 | 60
Recommended complementary curricular units
Prerequisites and co-requisites
I.Introduction to metabolism (Metabolism, anabolism, catabolism. Source of energy and redox reactions. Categories of metabolism. Metabolism and evolution.).
II.Aerobic metabolism (Digestion and absorption of glicids. Glycolysis. Fermentations associated to glycolysis. Glycogenogenesis and glycogenolysis. Gluconeogenesis. Krebs cycle. Pentose-phosphate cycle. Oxidative phosphorylation. Regulation.).
III.Photosynthesis (Light phase of photosynthesis (2 photosystems). ¿Dark phase¿.).
IV.Lipid metabolism (Digestion, absorption and transport. Beta-oxidation of fatty acids. Biosynthesis of fatty acids. Cholesterol metabolism.).
V.Aminoacid metabolism (Protein digestion. Transamination and deamination of aminoacids. Urea cycle. Cetoacid catabolism. Biosynthesis of aminoacids.).
VI.Transmembrane transport (Non mediated transport. Passive mediated transport. Active mediated transport using ATP. Active mediated transport using an ionic gradient.).
The discipline of Biochemistry II introduces the concept of metabolism and the main metabolic pathways. In particular a large part of the classes will be dedicated to the description of aerobic metabolism and associated pathways. By the end of the formation, the students should possess a clear knowledge of biochemical processes sustaining living organisms.
Knowledge, abilities and skills to be acquired
Knowledge and understanding of main metabolic pathways that are responsible for the production of cell energy and of biosynthesis pathways for most biomolecules, including aspects of evolutive relationships between different kinds of metabolism. Strengthening the students¿ abilities in techniques used in Biochemistry (preparation of reagent solutions, colorimetry, diferent techniques of liquid chromatography, calibration, spectrophotometry, extraction and purification of biomolecules like proteins and polysaccharides, electrophoresis, enzyme kinetics, database mining).
Teaching methodologies and assessment
Presential teaching with theoretical and laboratory classes: Theoretical classes are lectures that follow the defined syllabus. Laboratory classes are classes under the guidance of a teacher and are intended to practice the most frequent techniques.
Requirements to pass:
1) Continuous assessment
The final grade (NF) is the weighted average of the following components: Preparation of laboratory work 5% (component A), Performance and Attendance in the laboratory 5% (component B), reports of laboratory work 20% (component C), 2 Frequencies theoretical 70% (component D, each frequency is 35%). NF = A (5%) + B (5%) + C (20%) + D (70%).
2) Final exam:
Final exam covering all of the syllabus, taught in lectures, theoretical-practical and practical / laboratory.
Voet, D. & Voet. J.G. (2010) Biochemistry, (4th Ed). New York: John Wiley & Sons.
Halpern M, Freire A, Quintas A. (2008) Bioquímica Organização Molecular da Vida. Lidel
Berg, J.M. et al. (2010) Biochemistry (7th Ed). New York: Freeman and Company.
Devlin T. (2011) Textbook of biochemistry ; with clinical correlations. (7th Ed). Blucher
Weil, J.H. (2000) Bioquímica Geral (2ª Ed). Lisboa: Fundação Calouste Gulbenkian.