Part of this Programme
Level of Qualification|Semesters|ECTS
Bachelor; Master Degree | Semestral | 3
Year | Type of course unit | Language
1 |Mandatory |Português
Total of Working Hours | Duration of Contact (hours)
90 | 45
Recommended complementary curricular units
Prerequisites and co-requisites
I. Molecules and molecular processes associated with the Central Dogma of Molecular Biology: Nucleic acids, their structure, organization and function. Replication, transcription and protein synthesis in eukaryotes.
II. Gene expression regulation in eukaryotes: genetic and epigenetic mechanisms.
III. Genetic mutations: types of mutations, causes, consequences and DNA repair mechanisms.
IV. Organization of eukaryotic genomes and sources of genetic diversity: transposons, polymorphisms, repetitive DNA, SNP's, CNV's.
V. Techniques used in molecular biology: recombinant DNA technology, methods of molecular hybridization, DNA amplification techniques, techniques to analyze variations in DNA, technologies for genome manipulation.
VI. Introduction to the specificity of molecular biology laboratory and applications of molecular biology in Veterinary Medicine.
1) To learn about the structure and function of nucleic acids and the major processes associated with the Central Dogma of Molecular Biology.
2) To learn about the various processes of gene expression regulation in eukaryotes.
3) To learn about the fundamentals of genetic mutations, their classification, causes and consequences.
4) To learn about the organization of the eukaryotic genome, causes of genetic diversity and polymorphisms
5) To learn the fundamentals of the main techniques used to study nucleic acids and DNA manipulation and being able to perform them correctly
6) Develop a small research project where students perform some molecular biology techniques and use bioinformatics tools
7) Acquisition of scientific skills and critical attitude necessary to results analysis, interpretation and discussion. Acquisition of skills in team work, bibliographic research and communication of scientific information.
Knowledge, abilities and skills to be acquired
Students must be able:
- To understand the structure and function of nucleic acids and the main processes associated with the Central Dogma of Molecular Biology, regulation of gene expression and mutagenesis processes.
- To understand the theoretical concepts of the basic techniques of Molecular Biology used in Research and Diagnosis in Veterinary Medicine (nucleic acid extraction, PCR, RFLP and agarose gel electrophoresis) and acquire the ability to perform them correctly.
- To use and handle correctly the material used in a Molecular Biology laboratory (micropipettes, centrifuge, electrophoresis equipment, etc.)
- To develop a small research project where the molecular techniques already mentioned are integrated and acquire the scientific skills and attitudes necessary for the analysis, interpretation and discussion of the results obtained.
- Students should also have a first contact with bioinformatics tools
Teaching methodologies and assessment
The evaluation methodology follows the REAC-FMV-ULHT.
Theoretical: master classes with audiovisual media
- Continuous evaluation: 2 mini-tests (50% each)
- Final exam in case of no approval by continuous evaluation
Practical: students will develop a research project aiming skills acquisition and consolidation of some molecular biology techniques. Students will be stimulated to obtain information through bibliographic research, to enhance skills for results¿ analysis and critical discussion and to have an attitude of scientific rigor.
- Continuous evaluation: 2 group mini-quizz (12,50%), oral presentation, in group, of a research theme (20%), written test (62%) and continuous evaluation (assiduity, interest and behavior, 5,5%)
- Final exam in case of no approval by continuous evaluation. This could be oral or written.
Final UC evaluation: weighted average of theoretical (50%) and practical (50%) grades
- Carlos Azevedo. (2005). Biologia Celular e Molecular (5ª ed.). Lisboa, Porto, Coimbra: Lidel.
- Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K. & Walter, P. (2011). Fundamentos da Biologia Celular. (3ª ed.) Porto Alegre: Artmed
- Alberts, B., Johnson, A., Lewis, J., Morgan, D., Raff, M., Roberts, K. & Walter, P. (2014). Molecular Biology of the Cell. (6ª ed.) New York: Garland Science
- Lodish, H., Berk, A., Kaiser, C.A., Krieger, M., Bretscher, A., Ploegh, H., Amon, A. & Scott, M.P. (2016). Molecular Cell Biology (8ª ed.). New York: W.H.Freeman & Co Ltd.
- Karp, G., Iwasa, J. & Marshall, W. (2016). Cell and Molecular Biology - Concepts and Experiments. (8ª ed.). Hoboken: John Wiley & Sons
- Griffiths, A.J.F., Wessler, S.R., Carroll, S.B. & Doebley, J. (2015). An Introduction to Genetic Analysis. (11ª ed.). New York: W.H.Freeman & Co Ltd.
- Brown, T.A. (2016). Gene Cloning and DNA Analysis: An Introduction. (7ª ed.). Chichester: John Wiley & Sons Ltd.