Cell Migration Workshop

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This workshop will address the role of cell migration in different physiopathological processes, the different types of cell migration and the respective morphological, functional and molecular alterations involved.

We will also present the most commonly used cell-based techniques, including the wound-healing assay, chemotaxis, random single cell migration, cell invasion, as well as molecular tools and assays to dissect the mechanotransduction processes. Practical and laboratory classes will allow students to contact with key methodologies in the area.

Program

Lectures

• Mammalian cell migration: concepts 
  • Douglas M. Noonan, Università degli Studi dell’Insubria, Italy
• Strategies to quantify migratory phenotypes 
  • Ana Fernandes, CBIOS – Universidade Lusófona, Portugal
• Molecular mechanisms of cell migration: a technical approach 
  • Nuno Saraiva, CBIOS – Universidade Lusófona, Portugal
• Dietary compounds and cell migration: impact on health and disease
  • Claudia Cerella, Laboratoire de Biologie Moleculaire et Cellulaire du Cancer, Luxembourg

Practical Classes (hands-on)

• Collective cell migration assays
• Chemotaxis, and chemoinvasion
• Analysis of cell adhesion and cytoskeleton
• Data analysis with Image J