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Presentation
Presentation
Provide integrated training in the field of Applied Biocatalysis, emphasizing the various applications of enzymes at the industrial level; the particular requirements of those applications; and the platforms that allow its implementation, namely formulation of enzymes and enzymatic reactors
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Class from course
Class from course
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Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor | Semestral | 5
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Year | Nature | Language
Year | Nature | Language
3 | Mandatory | Português
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Code
Code
ULHT2710-17408
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Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
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Professional Internship
Professional Internship
Não
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Syllabus
Syllabus
Introduction to enzyme technology and representative exemples of applications. Enzyme structure and function. Activity and Stability of Enzymes. Principles of enzyme kinetics. Models of inhibition, denaturation and inactivation of enzymatic activity. Enzyme modification: chemical methods and genetic manipulation / protein engineering. Enzymatic formulations. Methods and supports for immobilizing enzymes. Effect of immobilization on conformation, apparent kinetics and stability of the biocatalyst. Biocatalysis in non-conventional media. Selection strategies of the reaction system. Reactors for free and immobilized biocatalysts. Fundamentals of design and operation analysis of enzymatic reactors. Strategies for selecting the type of reactor. Laboratory classes: based on model systems, the activity and stability of various enzyme formulations are compared, the influence of operational conditions on these parameters and the implementation in reactors in selected cases is addressed
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Objectives
Objectives
Provide integrated training in applied biocatalysis, highlighting: the various applications of enzymes, focusing on the production and processing of materials and their requirements; the platforms that allow its implementation, namely formulation of enzymes and enzymatic reactors; the potential of biocatalysis in the development of sustainable processes and framework in the circular bioeconomy. The student is to memorize basic concepts and know and be able to describe the strategies that allow the critical analysis of enzymatic systems and establish the rational basis for the design and implementation of these systems. The student should apply knowledge to: analyze concrete cases; identify/create the most suitable enzymatic formulation, mode of operation and reaction medium for a given process; characterize the formulation in terms of activity, productivity and stability; model and evaluate the performance of enzymatic reactors operating with free and immobilized enzymes
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Teaching methodologies and assessment
Teaching methodologies and assessment
Use of active methodologies, in particular provision of material prior to the class through platforms such as Moodle, with basic guidance on the subject. The referred materials include videos, audios, infographics, among others. Later in class, students ask questions, discuss the subject and compare answers Presentation and discussion of case studies, problem solving, individually or in groups, to encourage interaction and team spirit
Evaluation
Continuous evaluation
P1: average of 2 tests or global frequency during the semester (80%) + presentation of a seminar (20%):60% of the final grade
P2: reports, behavior in class and discussion: 40% of the final grade
Minimum passing grade for P1 and P2: 10 points in each
Failing approval by continuous assessment, the student can take an exam in the resit assessment period according to the assessment regulation in force. Minimum grade to pass: 10
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References
References
Bhaskar, A., Vidhya, V.G. (2021) Enzyme Technology. India: Mjp Publisher. ISBN-13 : 978-8180940019
Bennett, A. (2018) Enzyme Kinetics And Regulation. United Kingdom: ED-Tech Press. ISBN:978-1-83947-176-6
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Office Hours
Office Hours
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Mobility
Mobility
No
