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Presentation
Presentation
Componente Teórica - Conhecimento dos métodos e mecanismos de modificação genética usados em Biotecnologia. São exemplos em tecnologia do DNA recombinante o uso vetores e métodos de clonagem em procariotas e eucariotas e a obtenção de animais e plantas geneticamente modificados.
Componente Prática - Construção de uma biblioteca genómica de Lactobacillus plantarum num vector plasmídico, clonagem e expressão de genes, pesquisa de genes por complementação. Análise Bioinformática dos genes identificados.
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Class from course
Class from course
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Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor | Semestral | 5
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Year | Nature | Language
Year | Nature | Language
3 | Mandatory | Português
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Code
Code
ULHT2710-55
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Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
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Professional Internship
Professional Internship
Não
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Syllabus
Syllabus
- Enzymes to modify DNA and restriction enzymes. Cloning vectors: Plasmids, Phagemids, Cosmids, BAC, YAC.
- Genomic and cDNA libraries.
- Analysis of cloned genes.
- DNA sequencing.
- Directed mutagenesis and knockout.
- Protein engineering.
- DNA manipulation in microorganisms, plants and animals.
- Cloning in bacteria and fungus.
- Strategies to transfer genes to animal cells, bacterias and plants
- Inducible expression systems. Recombinant induction systems. Site-specific recombination.
- Production of useful molecules. Recombinant therapeutic proteins.
- Manipulation of metabolic pathways
- Gene edition
- Improvement of agronomic characteristics by genetic manipulation. Resistance to diseases; abiotic stress; quality improvement.
- Genetic manipulation to study, prevent and cure diseases. Models for human diseases. DNA vaccines. Gene therapy.
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Objectives
Objectives
The main objective of this module is to introduce the students to the concepts of techniques and tools used in the technology of recombinant DNA. To accomplish this, the fundaments and applications of methodologies will be addressed. Genetic engineering or recombinant DNA technology prompted the biotechnological research beyond the limits of traditional biology and biochemistry. The isolation of genes, its nucleotide sequencing and the study of the genetic expression level allowed the technology to be exploited to study the structure, behavior and gene activity in more detail. This technology allowed the obtaining improved individuals, manipulating metabolic pathways and the synthesis of new products
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Teaching methodologies and assessment
Teaching methodologies and assessment
The interpretation and reading of articles that include the methodologies, developments and state-of-the-art applications. This approach is considered as an experience in a dry lab.This work will be presented to the class.
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References
References
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Primrose, S.B. and Twyman, R.M. Principles of Gene Manipulation and Genomics. Seventh Edition. Blackwell Publishing, (2006), Oxford, UK.
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Lodish, H., Berk, A., Matsudaira, P., Kaiser, C.A., Krieger, M., Scott, M.P., Zipursky, L., Darnell, J. Molecular Cell Biology.. Fifth Edition. Scientific American Books, (2010) New York, USA.
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Videira, A. Engenharia Genética ¿ Princípios e Aplicações. LIDEL-edições técnicas, (2015), Lisboa, Portugal
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Office Hours
Office Hours
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Mobility
Mobility
No
