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Presentation
Presentation
The Biomolecular Spectroscopy course aims to provide students with knowledge of different areas of spectroscopy that are fundamental to the study and understanding of biomolecules. The course covers a range of spectroscopic techniques, namely ultraviolet-visible absorption spectroscopy, fluorescence, infrared spectroscopy, Raman spectroscopy, resonance Raman spectroscopy, circular dichroism, and EPR. This course plays a fundamental role within this study cycle, as it provides students with the theoretical and practical foundations required to understand and apply these different spectroscopic techniques. These skills can subsequently be further developed and deepened at the second-cycle level, according to the students' chosen area of specialization.
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Class from course
Class from course
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Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor | Semestral | 6
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Year | Nature | Language
Year | Nature | Language
2 | Mandatory | Português
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Code
Code
ULHT7037-16920
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Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
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Professional Internship
Professional Internship
Não
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Syllabus
Syllabus
1.Absorption spectroscopy in UV-visible. 2.Fluorescence. 3.Vibrational Spectroscopy (infrared, Raman and Ressonance Raman) 4.Circular Dichroism 5.Electron paramagnetic Ressonance spectroscopy.
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Objectives
Objectives
Biomolecules Spectroscopy will focus on the main spectroscopic techniques used in the characterisation of biomolecules. The aim is that by the end of the CU, students will have acquired an understanding of the main theoretical and experimental foundations (data analysis and interpretation) of absorption spectroscopies in the UV-visible, fluorescence, infrared, Raman resonance, circular dichroism and electron paramagnetic resonance (EPR). The aim is for students to understand the fundamentals of these techniques, their applications to the study of biomolecules and how to establish relationships between structural properties, macromolecular dynamics and function.
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Teaching methodologies
Teaching methodologies
In the practical component, students will produce 2 short videos (max. 2 min.) in which they will present a concept covered in the theoretical classes to different audiences. Introduction of extra support for students (using e-learning) for additional homework exercises and a flexible space for students to ask questions.
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References
References
Bandwell, Fundamentals of molecular spectroscopy, 4th edition. MacGraw-Hill Education 2016. Hames, G.G. Spectroscopy for the biological sciences, Willey Interscience 2005 Que, L. Physical methods in bioinorganic chemistry:Spectroscopy and magnetism. University Science Books Review papers related with the spectroscopic techniques studied will be available in moodle.
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Assessment
Assessment
Descrição dos instrumentos de avaliação (individuais e de grupo):
Descrição
Data limite
Ponderação
Avaliação teórica (50%de ponderação na nota final)
Avaliação contínua
Frequência 1
Frequência 2
Nota Final da avaliação teórica=média das notas das 2 Frequências
Para que haja aprovação à UC a média das notas das 2 Frequências tem de ser igual ou superior a 9,5
no decurso do semestre
50%
50%
Exame de recurso (teórica)*
*caso não tenha aprovação na avaliação contínua
Para que haja aprovação à UC a nota final do exame tem de ser igual ou superior a 9,5
No final de
semestre
100%
Avaliação prática (50%de ponderação na nota final)
Questionários (cinco)
Realização de videos (2 videos)
Para que haja aprovação à UC a nota da prática tem de ser igual ou superior a 9,5
No decurso do semestre
Igual ponderação entre os diferentes instrumentos de avaliação
Nota Final será a média das duas componentes(teóricas e práticas) Teorica e pratica têm a mesma ponderação, 50% cada componente. Os estudantes terão acompanhamento nas aulas práticas à realização dos questionários. Será também disponibilizada bibliografia adequada e os estudantes serão também encorajados a pesquisar eles próprios bibliografia a utilizar.
Para poder ter aprovação à UC é necessário que os alunos tenham frequência a 2/3 das aulas práticas.
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Mobility
Mobility
No
