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Presentation
Presentation
Animal Cell and Tissue Technology is a tool of modern biotechnology involving several disciplinary areas. Culture media, facilities for the culture of animal cells and tissues and various tools for cell characterization are covered, particularly in the area of human health. This curricular unit introduces the basics of animal tissue culture as well as the historical background, culture types, maintenance and characterization tools involved in this process. Furthermore, it also focuses on animal tissue culture facilities, on biosafety guidelines for cell culture in in vitro conditions and on ways to avoid contamination.
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Class from course
Class from course
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Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor | Semestral | 5
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Year | Nature | Language
Year | Nature | Language
3 | Mandatory | Português
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Code
Code
ULHT2710-17409
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Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
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Professional Internship
Professional Internship
Não
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Syllabus
Syllabus
Description of contents
- Introduction to Animal Cell and Tissue Technology
- Animal Cells - basic concepts
- Cloning and expression of Heterologous proteins in animal cells
- Cell metabolism and its control in culture
- Culture media for animal cells
- Pos-translational modifications of the recombinant proteins
- Mechanisms of proliferation and cell death
- Bioreactors for animal cells
- Monitoring and control of cell cultures
- separation of animal cells
- Cell therapy and stem cells
- Gene therapy
- Tissue engineering
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Objectives
Objectives
Introduce and develop basic concepts, addressing the main technological methodologies involving cell culture. Highlight the basic theoretical and instrumental principles, as well as laboratory applicability. Address the theoretical foundations of the methods, equipment and characteristics, use and analysis of results. These fundamentals allow the acquisition of skills for practical laboratory execution, making possible the identification of similarities and differences between methodologies, as well as the definition of strategies for minimizing potential risks. The laboratory practices allow the development of technical skills fundamental to the culture of animal cells and tissues.
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Teaching methodologies and assessment
Teaching methodologies and assessment
The innovative methodologies to support the teaching-learning process used are essentially: Project Based Learning; Flipped Classroom; Design Thinking.
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References
References
- Chawla H. S (2004) - Plant Biotechnology. A Practical Approach. Science Publishers.
- Freshney, R. I. (1994). ¿Culture of Animal Cells - A Manual of Basic Technique¿, 3rd Edition. Wiley-Liss New York.
- Gamborg, O.L. and G. C. Phillips (eds). (1995) - Plant Cell, Tissue and Organ Culture. Springer Lab Manual.
- Loyola-Vargas, Victor M, Felipe Vasquez (2005) - Plant Cell Culture Protocols. In Methods in Molecular Biology Series. Scientific and Medical Publishers.
- Maarten J., Crispeels & David E. Sadava (2003) - Plants, Genes and Crop Biotechnology 2th Edition. Jones and Bartlett Publishers.
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Office Hours
Office Hours
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Mobility
Mobility
No
