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Presentation
Presentation
The Cell and Tissue Engineering course (CU) aims to provide students with a solid understanding of the fundamental principles and practical applications of this interdisciplinary field, which combines engineering, cell biology, and medicine. The objective is to enable students to design and develop innovative solutions for tissue and organ regeneration or replacement using cells, biomaterials, and bioactive factors.
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Class from course
Class from course
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Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor | Semestral | 5
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Year | Nature | Language
Year | Nature | Language
3 | Mandatory | Português
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Code
Code
ULHT1706-12856
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Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
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Professional Internship
Professional Internship
Não
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Syllabus
Syllabus
Theoretical program contents Introduction to Animal Cell and Tissue Technology - basic concepts Separation of Animal Cells Isolation and Handling of Stem Cells, Primary Cultures, and Immortalization Culture Media for Animal Cells Cellular Metabolism and its Control in Culture Mechanisms of Cell Proliferation and Death 3D Cultures Gene Therapy Cloning and Heterologous Protein Expression in Animal Cells Bioreactors for Animal Cells Monitoring and Control of Cell Cultures Cell Therapy and Stem Cells Tissue Engineering Biomaterials for Tissue Engineering Laboratory Program Contents Thawing, Maintenance, and Freezing of Animal Cell Cultures Effect of Fetal Bovine Serum on the Growth of an Animal Cell Culture Effect of Medium on the Phenotype of Animal Cells
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Objectives
Objectives
The curricular unit (CU) of Cell and Tissue Engineering aims to empower students to develop innovative solutions for the manipulation of cells and tissues for biomedical purposes, such as tissue and organ regeneration. Thus, it is intended for students to acquire the following skills: Understand the types of cell cultures (stem cells, primary cells, and immortalized cultures) and the requirements necessary for their maintenance and manipulation. Understand the metabolic characteristics of animal cells, as well as their behavior in tissues and organs. Understand and identify methodologies for genetic modification of cells for cellular therapy and heterologous protein expression. Understand and identify methodologies for the use of animal cells in an industrial context (bioreactors). Understand, identify, and apply methodologies for obtaining 3D cultures, as well as the application of biomaterials for tissue engineering.
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Teaching methodologies and assessment
Teaching methodologies and assessment
The teaching-learning process includes theoretical explanations with interaction between the instructor and students, as well as project-based learning, flipped classrooms, and Design Thinking. Assessment is conducted continuously and consists of two components: the theoretical component (TC) and the laboratory component (LC). The assessment of the TC is carried out through two periodic tests or a comprehensive exam, along with a group project. The assessment of the LC involves continuous evaluation, which includes the completion of mini-questionnaires (30%), the preparation of reports (30%), a practical test (20%), and a theoretical test (20%). The final grade is calculated through a weighted average: 50% (TC) + 50% (LC), with a minimum passing grade of 9.5 in each component required for approval.
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References
References
Cape-Davies, A; Freshney, R.I.; Culture of Animal Cells (A manual of basic techniques and specialized applications), 8th edition. Wiley-Blackwell. 2021. ISBN: 1119513014 Vunjak-Novakovic, G.; Freshney, R. I. Culture of cells for tissue engineering (Culture of specialized cells), John Wiley & Sons Inc. 2006. ISBN: 9780471629351 Lonza, R.; Langer, R.; Vacanti, J.P.; Atala, A. Principles of tissue engineering, 5th edition. Academic Press. 2020. ISBN: 9780128184226
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Office Hours
Office Hours
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Mobility
Mobility
No
