Class Cell and Tissue Engineering

Animal Cell and Tissue Technology is a tool of modern biotechnology that encompasses multiple disciplinary areas. This course addresses culture media, facilities for animal cell and tissue culture, biosafety guidelines for in vitro cell culture, methods to prevent contamination, and various tools for cellular characterization, particularly in the context of human health. It also introduces the fundamentals of animal tissue culture, including its historical background, types of cultures, biotechnological applications, and tissue engineering.

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Description of contents Introduction to Animal Cell and Tissue Technology Animal Cells - basic concepts Cloning and expression of Heterologous proteins in animal cells Cell metabolism and its control in culture Culture media for animal cells Pos-translational modifications of the recombinant proteins Mechanisms of proliferation and cell death Bioreactors for animal cells Monitoring and control of cell cultures separation of animal cells Cell therapy and stem cells Gene therapy Tissue engineering

The teaching-learning process include theoretical explanations with interaction between teachers and students, based on interactive platforms such as SLIDO. Evaluation is conducted continuously and consists of two components: theoretical component (TC) and laboratory component (LC). The TC is assessed through quizzes performed during classes, two midterm exams, or a comprehensive exam, and a group project. The LC assessment consists of continuous evaluation (15%), and completion of reports (15%), discussion (30%), a practical test (20%), and a theoretical test (20%). The final grade is calculated using a weighted average: 50% (TC) + 50% (LC), and a minimum grade of 9.5 out of 20 is required in each component for passing. This comprehensive evaluation approach ensures a balanced assessment of students' performance in both the theoretical and practical aspects of the course. Students may opt for non-continuous evaluation, which consists of a final exam

Freshney, R. I. (1994). ¿Culture of Animal Cells - A Manual of Basic Technique¿, 3rd Edition. Wiley-Liss New York. Masters, John R W (ed.) (2023), Animal Cell Culture: A Practical Approach , https://doi.org/10.1093/oso/9780199637973.001.0001 Soccol C.R., Molento C. F. M., Reis G. G., Karp S. G. (2024), Cultivated Meat, ISBN: 978-3-031-55970-9