Class Bio-separations

Knowledge about biomolecule separation and purification techniques, processes and methodologies
Knowledge about the equipment used for biomolecule recovery and purification and the basic principles for its design

Theoretical
Introduction to biological product separation and recovery processes
Downstream processing concept: main steps and process sequence. Examples
Cell breakage processes: chemical, physical and mechanical
Solid-liquid separation processes: filtration, centrifugation, sedimentation, membrane processes.
Liquid-liquid extraction and leaching processes.
Chromatographic / Adsorption Processes
Supercritical Extraction
For each of the processes considered, the principles inherent to the separation method, the advantages
and limitations and the equipment used are identified, and design basics are introduced.
Practice
Cellular disruption (chemical, physical and mechanical methods) over prokaryotes and eukaryotes for
total protein recovery
Carotenoid recovery from carrot through leaching
Liquid-liquid extraction for salicylic acid recovery
Gel filtration chromatography for protein extract separation
Acetic acid adsorption on activated carbon

Use of active methodologies, in particular provision of material prior to the class through platforms such as Moodle, with basic guidance on the subject. The referred materials include videos, audios, infographics, among others. Later in class, students ask questions, discuss the subject and compare answers Presentation and discussion of case studies, problem solving, individually or in groups, to encourage interaction and group spirit

Assessment

Continuous Evaluation:

P1 - weighted average of two components: average of 2 tests or global frequency performed in

the semester (80%) + presentation of a seminar (20%): 60% of the final grade

P2 - based on scientific report, behavior in class and discussion: 40% of the final grade.

The minimum passing grade for P1 and P2 is 10 points.

Exam

If the student does not obtain approval by continuous assessment, the student can take an exam

 with a minimum grade of 10 to pas

• HARRISON, R.G.,TODD, P.W., RUDGE, S.R., PETRIDES, D.P. Bioseparations Science and Engineering (2nd ed.), Oxford: Oxford University Press, 2015. Hardcover (ISBN: 978-0195391817)
• BESCHKOV, V.N,  YANKOV, D. Downstream Processing in Biotechnology. De Gruyter, 2021.  ISBN: 9783110574005