-
Presentation
Presentation
In the field of biology, biochemistry and scientific research in general, molecular biology increasingly occupies a fundamental role. The great evolution of molecular knowledge and the development of laboratory techniques overlooking this area, become vital in areas as important as that of medicine. Basic knowledge in molecular biology will allow students to integrate in an easy way in any laboratory environment.
-
Class from course
Class from course
-
Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor | Semestral | 5.5
-
Year | Nature | Language
Year | Nature | Language
2 | Mandatory | Português
-
Code
Code
ULHT2532-3910
-
Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
-
Professional Internship
Professional Internship
Não
-
Syllabus
Syllabus
Part 1. Genome analysis 1. Genomes organization. Sequencing techniques: Sanger versus NGS (Next generation sequencing) 2. Molecular mechanisms of gene expression regulation 3. Molecular mechanisms of genetic recombination 4. Processes of genetic transfer and recombination in prokaryotes Part 2. Genomics analysis 5. Usage of genomic analysis techniques 6. Techniques for analysis of transcription process. Real time PCR vs RNAseq. 7. Alteration of gene expression - Epigenetics. DNA methylation. Histone modification and non-coding RNA (ncRNA). 8. Recombinant DNA technology Part 3. Fundamental cellular processes 9. Cellular signalling 10. Carcinogenesis Practical program 1. Basic rules of lab security, solution preparation and pipeting. 2. Basic techniques of nucleic acid manipulation 3. Construction of restriction maps: DNA digestion with restriction enzymes and result analysis. 4. PCR: Experiments preparation and optimization. 5. Recombinant DNA molecules construction: cloning process in bacteria
-
Objectives
Objectives
With this CU we intend to consolidate concepts previously acquired and which are fundamental to understand the molecular biology of the cell, and develop the main mechanisms of regulation of gene expression. It is intended with this CU to provide a solid foundation in the area of molecular biology, giving students the notion of the diversity of techniques and concepts that involve the subject and the great practical applicability that they have in the current practice of biology, biomedicine and even in the areas of clinical medicine. It is also intended to promote the acquisition of knowledge in the main techniques in molecular biology and theoretical foundations that support them and, above all, to promote the ability to develop a highly specialized lab work.
-
Teaching methodologies
Teaching methodologies
In addition to the weekly follow-up, students will have at their disposal in the e-learning moodle platform, discussion topics, additional articles that may be of interest to their training. It is intended in this way to create a great involvement and active participation of students in the CU, and a daily interest for the themes discussed. Lectures will be taught in person or by elearning using the Zoom platform whenever necessary.
-
References
References
- Alberts, Bray, Hopkin, Johnson, Lewis, Raff, Roberts and Walter (2013). Essential Cell Biology. 4th Ed. Garland Science. - Lodish, Berk, Kaiser, Krieger, Bretscher, Ploegh, Amon and Scott (2012). Molecular Cell Biology. 7th Ed. W. H. Freeman and Company. - Tropp, B.E. (2014). Principles of Molecular Biology. Jones & Bartlett Learning. - Weaver, R. (2011). Molecular Biology. 5th Ed. McGraw-Hill Education. - Williams, S.A., Slatko, B.E., McCarrey, J.R. (2007). Laboratory Investigation in Molecular Biology. Jones & Bartlett Learning.
-
Assessment
Assessment
Como estratégia de avaliação por frequência, os alunos serão submetidos a:
- Duas frequências teóricas (40%);
- Uma apresentação oral teórica no decurso das aulas (10%);
- Uma frequência prática de laboratório (50% da nota final da UC).
Caso não concluam a UC desta forma:
- podem ainda ser sujeitos a um exame final teórico e outro prático, tendo cada um peso de 50% na nota final.
A não realização de qualquer um dos componentes de avaliação, quer na parte teórica, quer na parte prática, implica a reprovação à avaliação contínua
Nesta disciplina não é permitida a utilização de ferramentas de Inteligência artificial para elaboração dos trabalhos escritos, apresentações, frequências ou exames finais.
No caso dos trabalhos escritos, os mesmos deverão ser apresentados oralmente e a nota obtida individualmente por cada aluno na sua apresentação, será a nota final do trabalho.
As part of the continuous assessment strategy, students will be evaluated through:
- Two theoretical assessments (40% of the final grade);
- One oral theoretical presentation during class sessions (10%);
- One practical laboratory assessment (50% of the final course grade).
If students do not successfully complete the course through continuous assessment:
- They may still be required to take a final theoretical exam and a final practical exam, each contributing 50% to the final grade.
Failure to complete any of the assessment components, whether theoretical or practical, will result in failure of the continuous assessment process.
The use of Artificial Intelligence tools is strictly prohibited for the preparation of written assignments, presentations, assessments, or final exams in this course.
For written assignments, students must present their work orally. The final grade for the assignment will correspond to the individual grade each student receives for their oral presentation.
-
Mobility
Mobility
No
