-
Presentation
Presentation
The CU Laboratory Techniques in Biochemistry aims to provide students with basic skills in laboratory work to enable the use of techniques common to all biochemistry laboratories. It aims to assist the student in the preparation of reports, automatic data acquisition, analysis and treatment of experimental data, graphical data presentation and methods of measuring some properties. It is a basic UC that serves to prepare students for more focused UCs on specific aspects of biochemistry. In this UC, students learn good laboratory practices associated with safe and correct practice. It provides a first approach to analysis and handling of data, preparation of solutions and basic chromatographic and spectroscopic techniques that will be used later in other UCs.
-
Class from course
Class from course
-
Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor | Semestral | 3.5
-
Year | Nature | Language
Year | Nature | Language
1 | Mandatory | Português
-
Code
Code
ULHT2532-16912
-
Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
-
Professional Internship
Professional Internship
Não
-
Syllabus
Syllabus
1. Safety in the laboratory. Laboratory notebook.
2. Basic principles of data analysis (2.1. Accuracy / precision, 2.2. Types of errors, 2.3. Significant
figures, 2.4. Statistics and data analysis)
3. Preparation of solutions.
4. Buffer solutions. Biological buffers.
5. UV-Visible absorption spectroscopy.
6. Protein quantification methods.
7. Chromatographic techniques. Basic principles.
8. Instrumentation and techniques (centrifugation, electrophoresis)
-
Objectives
Objectives
The CU of Laboratory Techniques in Biochemistry has as main objective that students become familiar with the most used and ubiquitous laboratory techniques in Biochemistry. Correct posture in the lab.,good safety practices and basic notions of data analysis and are pursued. Different techniques such asUV-visible absorption spectroscopy, protein quantification methods, chromatography, centrifugation andelectrophoretic techniques. At the end of the course, students should master these basic techniques atthe experimental level, which will be used in many courses in the area of of biochemistry and chemistry during their degree. Students should be able to prepare solutions (buffers), distinguish precision from
accuracy and use quantitative methods. Have a clear notion of detection limits and associated error, aswell as the precautions needed when handling different instruments (centrifuges, electrophoresis andspectrophotometers).
-
Teaching methodologies and assessment
Teaching methodologies and assessment
This CU is essentially practical and the practical work is directly related to the theoretical classes.
Students carry out group and individual work.
-
References
References
J.Martinho Simões, M. Castanho et a l, Guia do laboratório de Química e Bioquímica 2ª ed , Edt Lidel
K. Wilson and J. Walker, Principals and Techniques of Biochemistry and Molecular Biolog y,7th edition
Cambridge University PressR.Boyer, Biochemistry Laboratory 2nd edition Pearson
-
Office Hours
Office Hours
-
Mobility
Mobility
Yes
